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目的研究阿胶强骨口服液(donkey-hide glue reinforcing bone oral solution,DGRBOS)对SD大鼠血液25-羟基维生素D3(25-OH-VD3)、1,25-羟基维生素D3(1,25-(OH)2-VD3)和肝脏VDR基因表达的影响,探讨DGRBOS治疗骨质疏松的疗效机制。方法6月龄SD大鼠36只,随机分为A组(假手术组),B组(卵巢切除+生理盐水组),C组(卵巢切除+阿胶强骨口服液组),每组12只。6个月后取材检测。采用ELISA法对去势大鼠血清25-OH-VD3和1,25-(OH)2-VD3进行研究,用荧光定量PCR对肝脏VDR基因进行定量分析。结果C组(卵巢切除+阿胶强骨口服液组)血液中25-羟基维他命D3浓度和1,25-羟基维他命D3浓度与B组(卵巢切除+生理盐水组)比较明显增高,其中对25-羟基维他命D3浓度增高尤为明显,已接近A组(假手术组),P=0.002(P<0.01),差异有明显的统计学意义。荧光定量PCR(FQ-PCR)结果B组与C组相比,P=0.004(P<0.01),说明扩增效率的差异有明显的统计学意义。结论阿胶强骨口服液增加去势大鼠血液中25-OH-VD3、1,25-(OH)2-VD3的浓度,同时上调肝脏中VDR的表达水平,是阿胶强骨口服液治疗骨质疏松的机制之一。
Objective To study the blood glucose of 25-OH-VD3 and 1,25-hydroxyvitamin D3 (1,25-) in diabetic rats treated with donkey-hide gluing bone for bone oral solution (DGRBOS). OH)2-VD3) and the effect of liver VDR gene expression to investigate the therapeutic mechanism of DGRBOS in the treatment of osteoporosis. Methods Thirty-six SD rats aged 6 months were randomly divided into group A (sham operation group), group B (oophorectomy + saline group), and group C (oophorectomy + Ejiao Qianggu oral solution group) with 12 rats in each group. . 6 months after the detection. The serum 25-OH-VD3 and 1,25-(OH)2-VD3 in ovariectomized rats were studied by ELISA, and the liver VDR gene was quantitatively analyzed by real-time quantitative PCR. Results The concentration of 25-hydroxyvitamin D3 and 1,25-hydroxyvitamin D3 in blood of group C (ovariectomy + Ejiao Qianggu Oral Liquid) were significantly higher than those in group B (ovariectomy + saline group), of which 25- The increase in the concentration of hydroxyvitamin D3 was especially significant, and it was close to group A (sham), P=0.002 (P<0.01). The difference was statistically significant. Fluorescent quantitative PCR (FQ-PCR) results in group B compared with group C, P=0.004 (P<0.01), indicating that the difference in amplification efficiency was statistically significant. Conclusion Ejiao Qiangu oral liquid increases the concentration of 25-OH-VD3 and 1,25-(OH)2-VD3 in the blood of ovariectomized rats and up-regulates the expression level of VDR in the liver. One of the loose mechanisms.