论文部分内容阅读
本文报道了一种改进的测定血清褪黑激素的放射免疫方法.作者用Baker反相C-18层析柱从血清中抽提褪黑激素,发现上样的褪黑激素超过99%被保留在柱上,而97%以上能被洗脱到300微升甲醇中.用标准RIA的修订法测定血清抽提液中所含
In this paper, we report an improved radioimmunoassay for the determination of serum melatonin.The authors extracted melatonin from serum using a Baker reversed-phase C-18 column and found that over 99% of loaded melatonin was retained at While more than 97% can be eluted into 300 microliters of methanol.The standard RIA Amendment is used to determine the amount of serum contained in the serum extract