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目的 :探讨锗 Ge M1 0对黑色素瘤细胞增殖的作用 ,及其对高转移性黑色素瘤细胞与内皮细胞粘连的影响。方法 :用 3H- Td R掺入实验检测了 Ge M1 0对 A375黑色素瘤细胞增殖的影响 ;用细胞粘连抑制实验测定了 Ge M1 0对内皮细胞和 A375黑色素瘤细粘连的影响。结果 :Ge M1 0能抑制 A375黑色素瘤细胞 DNA合成 ,浓度为 1 80～ 30 0 μg/ml时抑制率最大 ,可达 73% ,作用时间 2 4 h各浓度 Ge M1 0抑制效果最强。Ge M1 0能部分阻断由L PS和 TNF刺激的内皮细胞和 A375的粘连 ,和对照组比较有显著性差异。结论 :Ge M1 0抗癌活性不仅表现在对肿瘤细胞的直接抑制增殖作用 ,而且可以抑制高转移性黑色素瘤细胞与内皮细胞粘连 ,从而抑制转移瘤形成
Objective: To investigate the effect of Ge Ge M1 0 on the proliferation of melanoma cells and its effect on the adhesion of highly metastatic melanoma cells to endothelial cells. METHODS: The effect of Ge M1 0 on the proliferation of A375 melanoma cells was detected by 3H-TdR incorporation assay. The effect of Ge M1 0 on the adhesion of A375 melanoma cells was determined by inhibition of cell adhesion. Results: Ge M1 0 could inhibit the DNA synthesis of A375 melanoma cells. The inhibitory rate of Ge M1 0 was the highest at concentrations of 180 ~ 300 μg / ml, reaching up to 73%. Ge M1 0 had the strongest inhibitory effect at 24 h. Ge M1 0 partially blocked the adhesion of endothelial cells stimulated by LPS and TNF to A375, which was significantly different from the control group. Conclusion: The antitumor activity of Ge M1 0 not only shows the direct inhibitory effect on proliferation of tumor cells, but also inhibits the adhesion of highly metastatic melanoma cells to endothelial cells and thus inhibits the formation of metastases