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选择增殖细胞核抗原 (PCNA)和血管内皮生长因子(VEGF)作为靶基因 ,设计针对 PCNA m RNA和 VEGFm RNA的两种反义核酸作用于人肝癌细胞株及裸鼠肝癌模型 ,观察人肿瘤细胞增殖情况及生物学行为的变化 ,及对裸鼠致瘤的抑制效应。1.增殖细胞核抗原反义核酸抑制人肝癌细胞体外增殖的
Two kinds of antisense nucleic acids targeting PCNA m RNA and VEGFm RNA were designed to act on human hepatocellular carcinoma cell lines and in nude mice hepatocellular carcinoma model with proliferating cell nuclear antigen (PCNA) and vascular endothelial growth factor (VEGF) as target genes to observe the proliferation of human tumor cells Changes in conditions and biological behavior, and on tumor suppressor effect in nude mice. 1. Proliferating cell nuclear antigen antisense nucleic acid inhibits human hepatoma cell proliferation in vitro