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目的研究大鼠肠系膜淋巴管运动及其瓣膜的动态特点；探讨ＮＯ对淋巴管的作用及ＮＯ在淋巴管及其支配神经的分布。方法用倒置显微镜和图像处理系统对肠系膜及其瓣膜进行动态观察和检测，并取肠系膜淋巴管、胸中段脊髓、延髓、脊神经节和腹腔神经节行ＮＡＤＰＳ－ｄ组化染色。ＮＡＤＰＨ－ｄ染色的肠乐膜淋巴管作电镜观察。结果淋巴管收缩频率５～１２次，平均为８．７±０．３次。淋巴管运动时瓣膜下游的淋巴管收缩幅度较瓣膜上游的淋巴管收缩幅度大 ２７．６％。施加 Ｌ－Ａｒｇ后淋巴管口径由２.４９±１. ２９象素增加到 ３． ２２± １.１８象素，淋巴管舒张 ３６．１％。淋巴管管径与两瓣膜间距离的相关关系ｒ＝０．２９７，呈低度相关。瓣膜的关闭和开放与淋巴管的收缩和舒张无明显规律，淋巴液的流动不因淋巴管的收缩而单方向流动。肠系膜淋巴管和瓣膜的内皮细胞以及其周围和附壁的神经纤维皆呈ＮＯＳ阳性反应。迷走神经背核、胸中部脊髓侧角、腹腔神经节和脊神经节神经元的胞浆及突起皆为ＮＯＳ阳性反应。电镜下淋巴管内皮细胞胞浆内ＮＯＳ反应产物，为致密颗粒构成的大小和形状不一的块状结构。结论实验结果提示瓣膜的开放和关闭除了机械因素外可能有其他调节因素。ＮＯ可舒张淋巴管，ＮＯ既是?
Objective To investigate the mesenteric lymphatic movement and the dynamic characteristics of the valve in rats. To explore the effect of NO on lymphatic vessels and the distribution of nitric oxide in lymphatic vessels and their dominant nerves. Methods The mesenteric and its valves were observed and detected by inverted microscope and image processing system. The mesentery lymphatic vessels, medial thoracic spinal cord, medulla oblongata, spinal ganglia and celiac ganglia were examined by NADPS-d staining. NADPH-d stained intestinal membrane lymphatic vessels for electron microscopic observation. Results The frequency of lymphatic contraction was 5 ~ 12 times with an average of 8.7 ± 0.3 times. Lymphatic vessel movement downstream of the valve when the contraction of the lymphatic vessels than the valve upstream of the contraction rate of 27.6%. Lymphatic vessel diameter increased from 2.49 ± 1. 29 pixels to 3 after L-Arg administration. 22 ± 1.18 pixels, lymphatic dilation 36.1%. The correlation between the diameter of the lymphatic vessel and the distance between the two valves was r = 0.297, showing a low degree of correlation. Valve closure and opening and contraction and relaxation of lymphatic vessels no obvious rule, the lymphatic fluid flow does not shrink due to lymphatic unidirectional flow. The mesenteric lymphatic vessels and the endothelial cells of the valve and the surrounding and adherent nerve fibers showed NOS positive responses. The vagus nerve dorsal nucleus, medullary midline lateral horn, celiac ganglion and spinal ganglion neurons in the cytoplasm and protuberance are all NOS positive reaction. Electron microscope lymphatic endothelial cells in the cytoplasmic NOS reaction products for the dense particles of the size and shape of the block structure. Conclusion The experimental results suggest that the valve opening and closing in addition to mechanical factors may have other regulatory factors. NO can relax lymphatic vessels, NO both?