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目的探讨抗辐灵对微波辐射致大鼠心脏损伤的保护作用。方法 100只二级雄性Wistar大鼠随机分为5组:正常对照组(N)、辐射对照组(R)、抗辐灵1 g/(kg/d)组(S)、2 g/(kg/d)组(M)及4 g/(kg/d)组(L)。抗辐灵药物组每日1次灌胃给予抗辐灵溶液,连续给药14 d,给药后7d采用30 mW/cm2微波辐射大鼠1次,辐射时间为10 min。于给药7 d(辐射后6 h)、停药后6 h(辐射后7 d)、停药后7 d(辐射后14 d)采用全自动生化检测仪检测血清乳酸脱氢酶(lactate dehydro-genase,LDH)和天门冬氨酸转氨酶(aspartate aminotransferase,AST),采用光镜和电镜观察心脏组织学和超微结构变化。结果 (1)给药7 d(辐射后6 h),R组血清中AST明显升高(P<0.05),心肌细胞轻度水肿;与R组相比,S、M及L组大鼠血清中LDH和AST活性、心脏组织学及超微结构均未见明显差异。(2)停药后6 h(辐射后7 d),R组大鼠血清中LDH和AST均明显升高(P<0.05),心肌纤维呈波浪状排列,线粒体肿胀,形态异常,血管内皮细胞胞饮小泡增加;S组上述指标与R组相似;M与L组大鼠血清中LDH和AST活性与R组相比明显降低(P<0.05);心肌纤维排列整齐;线粒体结构清晰,血管内皮细胞胞饮小泡明显减少。结论微波辐射可引起大鼠心肌酶异常和组织结构损伤;抗辐灵对微波辐射致大鼠心脏损伤具有保护作用;2 g/(kg/d)抗辐灵可能为防治微波辐射致大鼠心脏损伤的有效剂量。
Objective To investigate the protective effect of Kang Ling on rat heart injury induced by microwave radiation. Methods Two hundred male Wistar rats were randomly divided into five groups: normal control group (N), radiation control group (R), Radix Ginseng 1 g / (kg / d) group (S) / d) group (M) and 4 g / (kg / d) group (L). The anti-radiation drug group was given gavage once daily for 14 days, and the rats were irradiated once with 30 mW / cm2 microwave for 7 days. The irradiation time was 10 minutes. Serum lactate dehydrogenase (LDH) was detected by automatic biochemical analyzer at 7 d (6 h after irradiation), 6 h after dosing (7 d after irradiation) and 7 d after dosing (14 d after irradiation) (LDH) and aspartate aminotransferase (AST). The changes of cardiac histology and ultrastructure were observed by light microscope and electron microscope. Results (1) After 7 d administration (6 h after irradiation), serum AST was significantly increased (P <0.05) and mild myocardial edema in R group. Compared with R group, the serum levels of S, M and L LDH and AST activity, cardiac histology and ultrastructure showed no significant difference. (2) Serum LDH and AST in group R were significantly increased (P <0.05) at 6 h after irradiation (7 d after irradiation), myocardial fibers were arranged in a wavy pattern, mitochondria were swollen and morphologically abnormal, and vascular endothelial cells The above indexes in group S were similar to those in group R. The activities of LDH and AST in serum of rats in group M and L were significantly lower than those in group R (P <0.05); the myocardial fibers were arranged neatly; the structure of mitochondria was clear; Endothelial cell vesicles were significantly reduced. CONCLUSION: Microwave radiation can cause myocardial enzyme abnormalities and tissue damage in rats. Radiation-resistant spleen has a protective effect on heart damage induced by microwave radiation. Radix gavage at 2 g / (kg / d) may be protective against microwave radiation in rat heart Effective dose of injury.