以具有肿瘤特异性、p H响应型和细胞穿透肽性能的多肽H7K(R2)2为靶向配体,p H响应型脂质体为载体,采用薄膜水化法结合硫酸铵梯度法制备了多柔比星(DOX)脂质体[DOXPSL-H7K(R2)2],并考察其体外释放及对神经胶质瘤的靶向性和抗肿瘤活性。体外试验证实,H7K(R2)2修饰的p H响应型脂质体受到酸性环境的触发,能表现出特异性靶向于C6(大鼠C6胶质瘤细胞)和U87-MG(人成胶质细胞瘤细胞)细胞的性能,并释 H7K (R2) 2, a tumor-specific, pH-responsive, and cell-penetrating peptide, was used as a targeting ligand and pH-responsive liposome as a carrier. The membrane was hydrated with ammonium sulfate gradient Doxorubicin (DOX) liposomes [DOXPSL-H7K (R2) 2] and its in vitro release and targeting and anti-tumor activity to glioma were investigated. In vitro experiments confirmed that H7K (R2) 2-modified pH-responsive liposomes were triggered by an acidic environment and were shown to specifically target C6 (rat C6 glioma cells) and U87-MG Stem cell) cells, and release