论文部分内容阅读
建立适合肠上皮整复研究的IEC-6细胞迁移药理实验模型,并观察黄芪糖复合物对细胞迁移的影响。方法:设立不同的细胞接种密度、划痕后观察时间、辅助材料Matrigel浓度、血清饥饿处理法、细胞迁移抑制剂DF-MO(二氟甲基鸟氨酸)浓度等以考察模型的建立条件,并在已建立的模型上观察受试药的效果。结果:①细胞宜以4×105/mL接种6孔板;②宜划痕后24 h观察细胞迁移数;③5%Matrigel为适宜浓度;④血清饥饿可造成细胞迁移数明显减少,应使用含血清的培养液。⑤DFMO 2.5~5 mmol/L为抑制细胞迁移适宜浓度。⑥黄芪糖复合物及阳性药精脒能促进细胞迁移。结论:建立了适宜药理实验的IEC-6细胞迁移模型,在此模型上可反映受试药对细胞正常迁移及迁移抑制的药效作用。
The IEC-6 cell migration pharmacological model suitable for intestinal epithelial restoration was established and the effect of Astragaloside complex on cell migration was observed. Methods: The establishment of different cell seeding density, observation time after scratching, Matrigel concentration of auxiliary material, serum starvation treatment, concentration of DF-MO (difluoromethylornithine) And observed the effect of the test drug on the established model. Results: ① cells should be inoculated with 4 × 105 / mL 6-well plates; ② 24 h after scratching should be observed cell migration number; ③ 5% Matrigel for the appropriate concentration; ④ serum starvation can cause cell migration significantly reduced the use of serum Of the broth. ⑤ DFMO 2.5 ~ 5 mmol / L to inhibit the appropriate concentration of cell migration. ⑥ Astragalus sugar complex and positive drug amidine can promote cell migration. Conclusion: The model of IEC-6 cell migration which is suitable for pharmacological experiments is established. The model can reflect the pharmacodynamic effects of the tested drugs on the normal migration and migration inhibition of cells.