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１９７３年，Ｃｌａｒｋ等以褐对虾（Ｐｕｎａｅｕｕｓ ａｚｔｅｃｕｓ）为实验材料，将解剖的卵子和精子混合，首次在非鞭毛型不动精子的人工授精研究中获得受精卵，受精率为１０％［２］。１９８６年，Ｍｉｎ－ＮａｎＬｉｎ等在雌虾产卵时，将精子滴入产卵池中使卵子受精，
In 1973, Clark et al. Used Punaeuus aztecus as the experimental material, mixing the dissected eggs with sperm, and obtained fertilized eggs for the first time in artificial insemination studies of non-flagella fixed sperm at a fertilization rate of 10% [2]. In 1986, Min-NanLin and other spawning in the females, the sperm into the spawning pool egg fertilization,