本文提出不对称2D NOESY实验方法来测定完整的2D NOESY混合系数矩阵。理论研究表明,不对称2D NOESY实验信澡比要比传统2D NOESY的高,不对称2D NOESY峰强度矩阵是非对称的,因此可以只用不对称2D NOESY的非对角峰强度来求得完整的2D NOESY混合系数矩阵,避免测量大分子等复杂自旋系统的严重重叠的对角峰强度所引起的困难,而这是传统2D NOESY实验方法所无法作到的。本文最后应用数值模拟方法对理论研究结果进行了计算验证。 This paper presents an asymmetric 2D NOESY experimental method to determine the complete 2D NOESY mixing coefficient matrix. Theoretical studies show that asymmetric 2D NOESY experimental messengers are taller than conventional 2D NOESY meshes, asymmetric 2D NOESY peak intensity matrices are asymmetric, and therefore only the asymmetric 2D NOESY non-diagonal peak intensities can be used to obtain complete 2D NOESY mixing coefficient matrix avoids the difficulties caused by the measurement of the severely overlapping diagonal peak intensities of complex spin systems such as macromolecules, which traditional 2D NOESY experiments can not do. Finally, the numerical simulation method is used to verify the theoretical research results.