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目的了解攀枝花市主要食品中重要食源性致病菌的污染状况,为食品安全风险评估和预防食源性疾病提供科学依据。方法在全市5个区县抽查肉制品(包括生畜肉、生猪肉)、水产品、流动早餐3类食物样品共225份,按照2015年《国家食品污染物和有害因素风险监测工作手册》要求,运用VTEK2 COMPACT全自动细菌鉴定仪对样品进行病原菌鉴定,检测致病菌包括金黄色葡萄球菌、蜡样芽胞杆菌、沙门菌、副溶血性弧菌、霍乱弧菌、致泻性大肠埃希菌及单增李斯特菌。结果从225份样品中共检出食源性致病菌69株(30.67%),其中蜡样芽胞杆菌26株(47.27%),金黄色葡萄球菌20株(11.43%),沙门菌15株(6.67%),副溶血性弧菌6株(12.00%),致泻性大肠埃希菌2株(1.14%);3类食品中病原菌阳性率最高的是流动早餐(50.91%),其次为肉制品(28.33%),第三为水产品(14.00%)。结论攀枝花地区居民消费食品中存在着较为严重的食源性致病菌的污染,其中流动早餐是主要的污染食品;蜡样芽孢杆菌在流动早餐中污染较高,金黄色葡萄球菌在肉制品中污染严重。
Objective To understand the pollution status of important food-borne pathogens in Panzhihua City, and to provide a scientific basis for food safety risk assessment and prevention of food-borne diseases. Methods A total of 225 samples of meat products (including raw meat, raw pork), aquatic products and mobile breakfast were selected in 5 districts and counties of the city. According to the requirements of 2015 “National Food Contaminants and Hazard Risk Monitoring Work Manual” VTEK2 COMPACT automatic bacterial identification instrument was used to identify the pathogenic bacteria, including pathogenic bacteria including Staphylococcus aureus, Bacillus cereus, Salmonella, Vibrio parahaemolyticus, Vibrio cholerae, diarrheal Escherichia coli and Listeria monocytogenes. Results A total of 69 food-borne pathogens (30.67%) were detected from 225 samples, of which 26 were Bacillus cereus (47.27%), 20 (11.43%) were Staphylococcus aureus and 15 6 strains of Vibrio parahaemolyticus (12.00%) and 2 strains of diarrheal Escherichia coli (1.14%). The highest prevalence rate of pathogenic bacteria in three kinds of food was mobile breakfast (50.91%), followed by meat products (28.33%), the third is aquatic products (14.00%). Conclusion There are more serious pollution of food-borne pathogenic bacteria in consumer food in Panzhihua area, of which mobile breakfast is the main contaminated food, Bacillus cereus is highly contaminated in mobile breakfast and Staphylococcus aureus in meat products serious pollution.