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以路易斯安那鸢尾为试材,采用营养液培养方法,对铅(Pb)胁迫下路易斯安那鸢尾品种铅吸收量和非蛋白巯基(NPT)的变化等进行了研究。结果表明:路易斯安那鸢尾品种‘Professor Neil’对铅吸收和富集量相对较大且耐性最强。随着Pb浓度的升高,叶片、根状茎和根的Pb含量增加,增加量依次为根>根状茎>叶片。根在高浓度1 200mg/L Pb时达到最大,其含量为108 366mg/kg DW;根状茎在800mg/L Pb时达到最大,其含量为7 966mg/kg DW,呈先缓慢上升再下降的趋势;叶片在高浓度800mg/L Pb时达到最大,其含量为4 011mg/kg DW。随着Pb浓度的升高,根系耐受指数(IT)逐渐下降,在Pb含量大于600mg/L后存在显著性差异。根至茎转移率(R/R)呈先上升再下降的趋势,在800mg/L Pb处理时达到最大值10.02%。茎至叶转移率(R/L)呈先上升再下降的趋势,在600mg/L Pb处理时达到最大值57.78%。Pb胁迫下随着0~600mg/L浓度的升高和1~7d的时间推移,GSH和Cys含量呈增加的趋势并产生显著差异,尤其GSH起着重要的Pb解毒作用。
The results showed that the lead absorption and non-protein sulfhydryl (NPT) changes of L. iris varieties were studied by using nutrient solution culture method. The results showed that the Lewis Neisseria strain ’Professor Neil’ had a relatively large amount of lead absorption and enrichment and the strongest resistance. With the increase of Pb concentration, the contents of Pb in leaves, rhizomes and roots increased, followed by roots> rhizomes> leaves. The rhizome reached the maximum at the concentration of 1 200 mg / L Pb, and its content was 108 366 mg / kg DW. The rhizome reached the maximum at 800 mg / L Pb, with a content of 7 966 mg / kg DW, which firstly increased slowly and then decreased Trend; leaves in the high concentration of 800mg / L Pb maximum, the content of 4 011mg / kg DW. With the increase of Pb concentration, root tolerance index (IT) decreased gradually, and there was a significant difference after Pb content was more than 600mg / L. Root-to-stem transfer rate (R / R) increased first and then decreased, reaching the maximum of 10.02% at 800mg / L Pb treatment. The rate of stem to leaf translocation (R / L) increased first and then decreased, reaching the maximum of 57.78% at 600 mg / L Pb treatment. With the increase of 0-600 mg / L and the prolongation of 1-7 days, the content of GSH and Cys increased with the increase of Pb concentration, and the difference was significant. In particular, GSH played an important role in detoxification of Pb.