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本研究获得了三个分泌孕酮单克隆抗体的杂交瘤细胞株。这些高特异性抗体既和游离也和结合孕酮结合。这些抗体可和孕酮-牛血清白蛋白产生沉淀反应,但不沉淀牛血清白蛋白。它们可凝集覆盖有孕酮-牛血清白蛋白的羊红血球。它们的亲合力(Ka)在3～4.5×10~9M~(-1)之间。利用K(?)hlef 和Milstein 开创的杂交瘤技术制备激素单克隆抗体的工作开始不久,但已显示出巨大的潜力。利用这种技术获得大量均匀专一的孕酮抗体不仅有助于孕酮免疫测定的标准化,也将促进对孕酮在生殖过程中的作用的进一步了解。本文报道我们实验室利用杂交瘤技术获得的3个分泌孕酮单克隆抗体的细胞株。
Three hybridoma cell lines secreting monoclonal antibodies to progesterone were obtained in this study. These highly specific antibodies bind both to free and bound progesterone. These antibodies precipitated with progesterone-bovine serum albumin but did not precipitate bovine serum albumin. They can agglutinate sheep red blood cells covered with progesterone-bovine serum albumin. Their affinity (Ka) is between 3 ~ 4.5 × 10 ~ 9M ~ (-1). The work on the preparation of monoclonal antibodies to the hormones using the hybridoma technology pioneered by Köhlef and Milstein started soon but has shown great potential. The use of this technique to obtain a large number of uniformly-specific progesterone antibodies not only contributes to the standardization of progesterone immunoassays, but will also promote further understanding of the role of progesterone in the reproductive process. This article reports three cell lines secreting monoclonal antibodies to progesterone obtained by our laboratory using hybridoma technology.