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目的:采用超高效液相色谱法(UPLC)测定豌豆尖中类黄酮水解产物槲皮素和山奈酚的含量。方法:采用Waters ACQUITY UPLC BEH Shield RP18色谱柱(100 mm×2.1 mm,1.7μm),以乙腈-0.1%冰醋酸水溶液(60:40)为流动相,流速为0.4 m L/min,槲皮素检测波长为371 nm,山奈酚检测波长为366 nm,柱温为30℃,进样量2.0μL。结果:槲皮素和山奈酚在1.5 min时间内达到基线分离,分别在(0.03~0.18)mg/m L、(0.015~0.15)mg/m L范围内与峰面积线性关系良好,平均回收率分别为101.37%、100.64%,RSD分别为0.84%、0.37%。结论:该方法快速、准确、稳定,可用于豌豆尖中槲皮素和山奈酚的含量测定。
OBJECTIVE: To determine the contents of quercetin and kaempferol, the flavonoid hydrolyzate of pea tip, by ultra performance liquid chromatography (UPLC). METHODS: A Waters ACQUITY UPLC BEH Shield RP18 column (100 mm × 2.1 mm, 1.7 μm) was used with a mobile phase of acetonitrile-0.1% glacial acetic acid (60:40) at a flow rate of 0.4 m L / min. Quercetin The detection wavelength was 371 nm, the detection wavelength of kaempferol was 366 nm, the column temperature was 30 ℃ and the injection volume was 2.0 μL. Results: Quercetin and kaempferol achieved baseline separation within 1.5 min, and showed good linear relationship with peak area in the range of (0.03-0.18) mg / m L and (0.015-0.15) mg / m L, respectively. The average recovery Respectively, 101.37%, 100.64%, RSD were 0.84%, 0.37%. Conclusion: The method is rapid, accurate and stable and can be used for the determination of quercetin and kaempferol in peas.