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用辣根过氧化物酶标记ＭｃＡｂＦ（ａｂ＇）_２、ＭｃＡｂＬＶ４８Ａ、ＭｃＡｂ３Ｄ_８和ＭｃＡｂＡ_（３５），分别对２７例ＥＨＦ患者尿液中融合细胞（ＦＣ）进行免疫酶染色。结果ＭｃＡｂＦ（ａｂ＇）_２检出率为２７／２７（１００％）；ＭｃＡｂＬＶ４８Ａ为１９／２７（７０．３７％）；ＭｃＡｂ３Ｄ_８为２０／２７（７４．０７％）；ＭｃＡｂＡ_（３５）为２／２７（７．４１％）。人胚肾细胞感染ＥＨＦ病毒后，在偏酸性（ｐＨ６．０）条件下可以产生特异性细胞融合现象，并经电镜证实了细胞之间膜的融合。此结果表明ＥＨＦ病毒可以导致人肾细胞融合，ＥＨＦ患者尿液中ＦＣ的形成可能与ＥＨＦ病毒膜蛋白在宿主细胞膜上的表达有关。
The horseradish peroxidase-labeled McAbF (ab ’) _2, McAbLV48A, McAb3D_8 and McAbA_ (35) were used to detect the immunofluorescence staining of fusion cells (FC) in 27 EHF patients. Results The detection rate of McAb F (ab ’) 2 was 27/27 (100%), McAbLV48A was 19/27 (70.37%), McAb3D_8 was 20/27 (74.07%), McAbA 35 was 2 / 27 (7.41%). After human embryo kidney cells were infected with EHF virus, specific cell fusion could be produced under the condition of partial acidity (pH6.0), and the fusion of cells was confirmed by electron microscopy. This result indicates that EHF virus can cause human renal cell fusion, and the formation of FC in the urine of EHF patients may be related to the expression of EHF virus membrane protein on the host cell membrane.