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利用新生牛肝脏，采用改良Ｌａｂｒｅｃｑｕｅ法提取肝再生刺激因子（ＨＳＳ），并应用非解离聚丙烯酰胺凝胶电泳对其进行纯化，ＳＤＳ－聚丙烯酰胺凝胶电泳证实其分子量约为１５０００道尔顿。经过纯化，其杂蛋白含量降低近９５０倍。
Hepatic regeneration was used to extract hepatic regeneration stimulating factor (HSS) by using modified Labrecque method and purified by non-dissociation polyacrylamide gel electrophoresis. SDS-polyacrylamide gel electrophoresis confirmed its molecular weight was about 15000 Dole pause. After purification, its hybrid protein content decreased nearly 950 times.