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目的研究中药复方制剂GC颗粒治疗功能性子宫出血的机制。方法选用50只正常雌性ICR小鼠,随机分成5组(每组10只):正常对照组,益妇止血丸组及高、中、低剂量GC颗粒组。采用断尾法及毛细波管法分别测定出凝血时间,观察GC颗粒对正常小鼠出、凝血时间的影响。选用40只早孕SD大鼠,以灌服米非司酮和米索前列醇造成不完全流产模型,并随机分成5组(每组8只):模型对照组,益妇止血丸组及高、中、低剂量GC颗粒组。出血时间采用毛细玻管法,凝血时间采用剪尾法测定,子宫出血量采用酶标仪对植入阴道棉球的血液浸渍液进行测定,观察GC颗粒对该模型大鼠出凝血时间及子宫出血量的影响。结果与正常对照组比较,GC颗粒中、大剂量组(8g/kg、16 g/kg)能明显缩短小鼠出、凝血时间(P均<0.01),与益妇止血丸组无明显差异(P均<0.05);与模型对照组比较,GC颗粒三个剂量组(2 g/kg、4 g/kg、8 g/kg)均能明显减少不完全流产大鼠子宫出血量(P均<0.05),与益妇止血丸组无明显差异(P均>0.05)。结论中药复方制剂GC颗粒可有效减少实验大鼠子宫出血量,其治疗功能性子宫出血的机制可能与其具有止血作用有关。
Objective To study the mechanism of GC granule in treating compound dysfunctional uterine bleeding. Methods Fifty normal female ICR mice were randomly divided into 5 groups (10 in each group): normal control group, Yishenhexue pill group and high, medium and low dose GC particles group. Closing method and capillary tube method were used to determine the clotting time, observe GC particles on the normal mouse clotting time. Forty pregnant rats were randomly divided into 5 groups (8 rats in each group): model control group, YFZH group and high, Medium and low dose GC particles group. Bleeding time using capillary tube method, clotting time using the tail cut method, uterine bleeding using a microplate reader implanted in the vaginal cotton ball blood immersion was measured to observe the GC particles of the model clotting time and uterine bleeding The impact of volume. Results Compared with the normal control group, GC and medium dose groups (8g / kg, 16g / kg) significantly shortened the clotting and clotting time (P <0.01) P <0.05). Compared with the model control group, the GC granule three doses (2 g / kg, 4 g / kg, 8 g / kg) could significantly reduce the uterine bleeding in incomplete abortion rats (all P < 0.05), no significant difference with Yifu hemostatic pill group (all P> 0.05). Conclusion GC granule of traditional Chinese medicine compound preparation can effectively reduce the amount of uterine bleeding in experimental rats, and its mechanism of treating functional uterine bleeding may be related to its hemostatic effect.