论文部分内容阅读
为了更好地在荔枝杂交育种中判别真杂种,以荔枝两个人工杂交群体‘雪怀子’ב桂味’和‘雪怀子’ב焦核三月红’的F1代为材料,利用EST-SSR标记进行真假杂种鉴定,创建作图群体,并对两个群体进行遗传多样性分析。结果表明,分别采用4对引物组合即可实现对两个杂交群体F1代单株100%的鉴定,两个群体的159个单株均为真杂种;且两个群体后代叶片形态存在变异,基因型上产生了不同于亲本的扩增谱带。经聚类分析发现,群体‘雪怀子’ב桂味’113个F1单株被聚为6大类(相似性系数0.68),63.72%(72株)后代与父本聚为一类,28.3%(32株)单株与双亲距离较远,推测这些单株中出现了较大的遗传重组或变异。在相似系数0.642处,‘雪怀子’ב焦核三月红’组合的46株杂种后代分为两大类,该组合大部分单株(60.87%)与母本亲缘关系较近,具有偏母本遗传倾向。可见,EST-SSR标记适合荔枝真假杂种鉴定,两个F1作图群体的创建为荔枝遗传连锁图谱构建奠定基础,同时为荔枝的品种改良积累材料。
In order to better discriminate true hybrids in hybridization of litchi, F1 hybrids of ’Xue Wei’ × ’Gui Wei’ and ’Xue Wai Zi’ × ’Jiao Nian March Red’ of two artificial litchi hybrids were used as materials, EST-SSR markers for identification of true and false hybrids, creating mapping groups, and genetic diversity analysis of two groups. The results showed that four pairs of primer combinations were used to identify 100% of the F1 hybrids in two crossbred groups. 159 of the two groups were all true hybrids. The resulting amplified bands differ from the parental ones. According to the cluster analysis, 113 F1 plants of ’Xuehuaizi’ × ’Guiwei’ were clustered into 6 groups (similarity coefficient 0.68), 63.72% (72 strains) 28.3% (32 plants) were far away from their parents, suggesting that a large genetic recombination or variation occurred in these plants. At the similarity coefficient of 0.642, 46 hybrid offspring of ’Xue-qiao’ × ’Jiao-san-March red’ were divided into two groups. Most of the hybrids (60.87%) had a close genetic relationship with the female parent, Partial mother genetic predisposition. It can be seen that the EST-SSR marker is suitable for the identification of true and false litchi hybrids, and the establishment of two F1 mapping populations lays the foundation for constructing the genetic linkage map of litchi and simultaneously accumulates materials for improving the variety of litchi.