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以沙芥无菌实生茎尖为外植体,探索了光照强度对茎尖培养的影响,研究了植物生长调节剂6-BA与IBA的不同浓度组合对沙芥幼苗短缩茎腋芽诱导和腋芽增殖的影响,同时研究了消毒方法对田间沙芥萌动腋芽增殖的影响。结果表明:最适宜茎尖分生组织生长的光照强度为3 600lx;最佳诱导腋芽萌发并进行伸长生长的植物生长调节剂组合为6-BA 5.0mg/L,IBA0.5~1.0mg/L,腋芽诱导率达93.3%~96.7%,平均诱导芽数为4.2~4.3;最适宜腋芽增殖的植物生长调节剂浓度组合为6-BA 5.0mg/L+IBA 1.0mg/L,丛生芽诱导率达93.1%,增殖系数达3.44;田间的萌动腋芽经70%酒精浸泡3~5s与0.1%升汞震荡10~12min,二者交叉消毒效果最理想,污染率为10%,成活率达到85%,褐化率只有20%,但外植体生长缓慢,生长周期长,增殖率极低,不适宜进行增殖培养。
In order to explore the effect of light intensity on shoot tip culture, we studied the effects of different concentrations of 6-BA and IBA on the induction of axillary buds and the formation of axillary buds Proliferation of the same time, at the same time studied the disinfection method on the field of Arabidopsis adventitious bud proliferation. The results showed that the optimum light intensity for shoot apical meristem culture was 3600lx. The optimum combination of plant growth regulator with 6-BA 5.0mg / L and IBA 0.5-1.0mg / L, the induction rate of axillary bud was 93.3% ~ 96.7%, the average number of induced buds was 4.2 ~ 4.3. The optimal concentration of plant growth regulators for axillary bud proliferation was 6-BA 5.0 mg / L + IBA 1.0 mg / L, The rate of up to 93.1% and the multiplication coefficient up to 3.44. The germination axillary bud in field was soaked in 70% alcohol for 3-5 s with 0.1% mercuric chloride for 10-12 min. The cross-disinfection effect was the best with the contamination rate of 10% and the survival rate of 85 %, Browning rate of only 20%, but the explants grow slowly, the growth cycle is long, the proliferation rate is very low, not suitable for proliferation and culture.