采用超临界流体萃取法（ＳＦＥ）提取中药何首乌中的蒽醌类成分，并用ＲＰ－ＨＰＬＣ法进行分离测定。在 ＳＦＥ中，通过对萃取条件的考察，确定萃取压力为 ４２ ＭＰａ，温度 ７０℃，改性剂量 ０．４ｍＬ，静态萃取时间 ５ｍｉｎ及动态萃取体积 ５ｍＬ。 ＲＰ－ＨＰＬＣ中，以Ｃ_８为固定相，甲醇－０． ２％磷酸溶液（７０：３０）为流动相，检测波长 ４３７ｎｍ。本法准确可靠，何首乌中各成分的平均回收率分别为大黄酸９９．１％（ＲＳＤ ２．０％）、大黄素 ９８．２％（ＲＳＤ１．１％）、大黄酚 １００．７％（ＲＳＤ ２．６％）和大黄素甲醚９９．５％（ＲＳＤ ０．９％）。 Supercritical fluid extraction (SFE) was used to extract the anthraquinones from Radix Polygoni Multiflori Thunb. And used RP-HPLC method for separation and determination. In the SFE, through the examination of the extraction conditions, it was determined that the extraction pressure was 42 MPa, the temperature was 70°C, the modification dose was 0.4 mL, the static extraction time was 5 min, and the dynamic extraction volume was 5 mL. In RP-HPLC, C_8 is used as the stationary phase and methanol is -0. A 2% phosphoric acid solution (70:30) was used as the mobile phase and the detection wavelength was 437 nm. The method is accurate and reliable. The average recoveries of the components in Radix Polygoni multiflori are 99.1% (RSD 2.0%), 98.2% (RSD 1.1%), and 100.7% (RSD) of rhein. 2.6%) and 99.5% of rhubarb methyl ether (RSD 0.9%).