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目的探究Ki-67核抗原(Ki-67)、P-糖蛋白(P-gp)在宫颈癌新辅助化疗前后的的变化,分析其与化疗疗效及敏感性之间的相关性,为新辅助化疗的敏感性指标的确定提供理论基础。方法随机挑选来永康市妇幼保健院行宫颈癌新辅助化疗的Ⅰb2~Ⅱb期宫颈癌患者50例,采用免疫组化的方法检测化疗前后宫颈组织中Ki-67、P-gp的表达,计算并分析其表达变化及与化疗效果和敏感性之间的相关性。结果宫颈癌新辅助化疗的总体有效率为86%,稳定的有7例,无患者表现为进展;Ki-67化疗前的阳性表达率为90.00%,显著高于化疗后的65.00%,差异具有统计学意义(χ2=23.67,P<0.05),P-gp化疗前阳性表达率为56.00%,化疗后为85.00%,差异具有统计学意义(χ2=21.98,P<0.05);化疗前Ki-67阳性的患者化疗有效率为91.11%,显著高于Ki-67阴性的化疗有效率(χ2=9.67,P<0.05),而化疗前P-gp阳性的患者化疗有效率为39.29%,P-gp阴性的有效率100.00%,差异也具有统计学意义(χ2=10.85,P<0.05)。Ki-67和P-gp的炎性表达呈现负相关关系(r=0.5872,P<0.05)。结论宫颈癌新辅助化疗后与化疗前相比Ki-67阳性表达率降低,P-gp阳性表达率升高,可为临床治疗提供依据。
Objective To investigate the changes of Ki-67 and P-gp in cervical cancer before and after neoadjuvant chemotherapy, and to analyze the correlation between Ki-67 and curative effect and sensitivity of chemotherapy Chemotherapy sensitivity index to provide a theoretical basis. Methods Fifty patients with stage Ⅰb2 ~ Ⅱb cervical cancer who underwent neoadjuvant chemotherapy for cervical cancer were selected randomly from Yongkang MCH center. The expressions of Ki-67 and P-gp in cervical tissues before and after chemotherapy were detected by immunohistochemistry Analysis of its expression changes and the correlation between the efficacy and sensitivity of chemotherapy. Results The overall effective rate of neoadjuvant chemotherapy for cervical cancer was 86%, stable in 7 cases and no progress in patients. The positive expression rate of Ki-67 before chemotherapy was 90.00%, significantly higher than 65.00% after chemotherapy, the difference was Statistical significance (χ2 = 23.67, P <0.05), the positive expression rate of P-gp before chemotherapy was 56.00% and 85.00% after chemotherapy, the difference was statistically significant (χ2 = 21.98, P <0.05) The positive rate of chemotherapy in 67 positive patients was 91.11%, which was significantly higher than that of Ki-67 negative patients (χ2 = 9.67, P <0.05), while the positive rate of chemotherapy in patients with positive P-gp was 39.29% gp-negative efficiency of 100.00%, the difference was statistically significant (χ2 = 10.85, P <0.05). The inflammatory expression of Ki-67 and P-gp showed a negative correlation (r = 0.5872, P <0.05). Conclusions After neoadjuvant chemotherapy for cervical cancer, the positive rate of Ki-67 expression is lower than that before chemotherapy, and the positive rate of P-gp expression is increased, which may provide the basis for clinical treatment.