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目的:研究三黄泻心汤水提取液舒血管作用的可能机制。方法:记录苯肾上腺素(PE)和KCl预收缩的离体大鼠主动脉环张力变化,观察三黄泻心汤水提取液舒血管作用及不同工具药对舒血管作用的影响。结果:三黄泻心汤水提取液(0.1,0.3,0.5mg/ml)对PE(1.0μmol.L-1)和KCl(50mmol.L-1)预收缩的大鼠主动脉环均有非内皮依赖的、浓度依赖性的舒张作用。对PE(1.0μmol.L-1)预收缩的去内皮血管环,三黄泻心汤水提取液(0.5mg/ml)呈舒张作用,分别用一氧化氮合酶抑制剂左旋硝基精氨酸甲酯(L-NAME)(0.1mmol.L-1)和鸟苷酸环化酶抑制剂亚甲蓝(MB)(10μmol.L-1)预处理无明显影响。在无钙营养液(含EGTA)环境下,三黄泻心汤预处理对苯肾上腺素(PE)收缩有明显抑制作用。结论:三黄泻心汤有浓度依赖性的血管舒张作用,此作用即不依赖血管内皮,又和内皮源性的舒张因子NO无关,可能与抑制血管平滑肌细胞内质网储存钙的释放有关。
Objective: To study the possible mechanism of the vasodilatory effect of Sanhuang Xiexin Decoction extract. Methods: The changes of the aorta ring tension of phenylephrine (PE) and KCl precontracted rat were recorded. The vasodilative effect of Sanhuang Xiexin Decoction extract and the effect of different tools on the vasodilatory effect were observed. Results: Sanhuang Xiexin Decoction extract (0.1, 0.3, 0.5 mg/ml) had non-endothelialization on PE (1.0 μmol.L-1) and KCl (50 mmol.L-1) precontracted rat aortic rings. Dependent, concentration-dependent relaxation. Pre-contracted PE (1.0 μmol.L-1) decapsulated vascular loops and Sanhuang Xiexin Decoction (0.5 mg/ml) were shown to be vasodilating. Nitric oxide synthase inhibitor L-nitro arginine was used. Methyl ester (L-NAME) (0.1 mmol.L-1) and guanylate cyclase inhibitor methylene blue (MB) (10 μmol.L-1) pretreatment had no significant effect. Under the environment of calcium-free nutrient solution (containing EGTA), pretreatment with Sanhuang Xiexin Decoction significantly inhibited the contraction of phenylephrine (PE). Conclusion: Sanhuangxiexin decoction has a concentration-dependent vasodilatation effect, which is independent of vascular endothelium, and is not related to endothelium-derived relaxing factor NO, and may be related to inhibiting the release of calcium from the endoplasmic reticulum of vascular smooth muscle cells.