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目的探讨氟对大鼠生精细胞Fas蛋白表达影响。方法将30只4～5周龄雄性Wistar大鼠随机分为对照组、低剂量组、高剂量组。分别按0、10和20mg/kg染毒剂量隔日灌胃氟化钠(NaF)染毒39天,采用酸浸-氟离子选择电极法测定睾丸氟含量,用放射免疫法测定大鼠血清睾酮含量,用免疫组化(ICH)检测睾丸中Fas蛋白表达情况,同时镜检成熟精子质量。结果各染氟组大鼠睾丸氟含量均高于对照组(P<0.05)且随染毒剂量的增加而逐渐升高;各染氟组大鼠血清睾酮含量低于对照组(P<0.05),且随着染毒剂量的增加,睾酮含量逐渐降低;各染氟组大鼠生精细胞Fas蛋白阳性表达率高于对照组(P<0.05),且随着染毒剂量的增加,Fas蛋白阳性表达率逐渐升高;各染毒组大鼠精子数量和精子活动率低于对照组(P<0.05);精子畸形率高于对照组(P<0.05)。结论染氟雄性大鼠血清睾酮水平降低,激活了生精细胞Fas/FasL系统,从而导致生精细胞损伤。
Objective To investigate the effect of fluoride on the expression of Fas protein in rat spermatogenic cells. Methods Thirty male Wistar rats aged 4-5 weeks were randomly divided into control group, low dose group and high dose group. Rats were treated with sodium fluoride (NaF) at doses of 0, 10 and 20 mg / kg every other day for 39 days. The content of testicular fluorine in the testis was determined by acid leaching-fluoride ion selective electrode method. The level of serum testosterone The expression of Fas protein in testis was detected by immunohistochemistry (ICH), and the quality of mature sperm was examined microscopically. Results The content of fluorine in testis of rats in each fluoride group was higher than that in control group (P <0.05), and increased gradually with the increase of exposure dose. The content of serum testosterone in each group was lower than that in control group (P <0.05) , And the testosterone content gradually decreased with the increase of exposure dose. The positive rate of Fas protein expression in spermatogenic cells in each group was higher than that in control group (P <0.05). With the increase of exposure dose, Fas protein (P <0.05). The rate of sperm deformity was higher than that of the control group (P <0.05). Conclusion The level of serum testosterone in the male rats with fluorosis decreased, activating the Fas / FasL system of spermatogenic cells, resulting in the damage of spermatogenic cells.