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对斑点嗜蓝孢孔菌乙醇提取物进行分级萃取,利用柱层析技术得到6个单体化合物。经核磁数据比对为β-谷甾醇(1)、麦角甾醇(2)、麦角甾-7,22-二烯-3β-醇(3)、5,8-过氧麦角甾-6,22-二烯-3β-醇(4)、3-乙酰齐墩果酸(5)、白桦脂醇(6)。采用Alamar Blue法检测各有机相及单体化合物对NCI-H460人非小细胞肺癌细胞增殖的影响,用DPPH自由基清除法检测其抗氧化活性。利用荧光光谱及紫外-可见光光谱研究方法在模拟人体生理条件下,研究了斑点嗜蓝孢孔菌水提物与人纤维蛋白原的相互作用,实验结果表明,斑点嗜蓝孢孔菌水提物与人纤维蛋白原的相互形成基态复合物导致人纤维蛋白原内源荧光猝灭。
The macroporous ethanol extract of Plastiporus sp. Was fractionated, and 6 monomer compounds were obtained by column chromatography. Comparison of the nuclear magnetic resonance data for β-sitosterol (1), ergosterol (2), ergosta-7,22-diene-3β- 3β-ol (4), 3-acetylene oleanolic acid (5), betulin (6). Alamar Blue method was used to detect the effect of each organic phase and monomer compound on the proliferation of NCI-H460 human non-small cell lung cancer cells. The anti-oxidative activity was detected by DPPH free radical scavenging assay. Fluorescence spectroscopy and UV-Vis spectroscopic methods were used to study the interaction between water extract of Pseudomonas aeruginosa and human fibrinogen under simulated human physiological conditions. The experimental results showed that the water extracts of Pseudomonas aeruginosa The formation of a ground state complex with human fibrinogen leads to endogenous fluorescence quenching of human fibrinogen.