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目前在临床病理诊断及病理、组胚等基础教学中,一般沿用石蜡切片和苏木素伊红(HE)染色,在其操作全过程中多以苯类溶剂(苯、二甲苯、甲苯)作为煤浸、脱蜡和透明溶剂,但苯的毒性作用对于人体肝脏、造血机能及中枢神经系统等均有不同程的损害,长期接触可以发生慢性苯中毒。为了减少苯的刺激,我们在参考同行的经验与报道的基础上,经过多年的探索、实践,自1980年以来用正丁醇作组织脱水兼透明
At present, in the basic teaching of clinicopathological diagnosis, pathology, and embryos, paraffin sections and hematoxylin-eosin (HE) staining are commonly used, and benzene solvents (benzene, xylene, and toluene) are often used as coal leaching during the entire operation process. , dewaxing and transparent solvents, but the toxic effects of benzene on the human liver, hematopoietic function and central nervous system, etc. have different course of damage, long-term exposure can occur in chronic benzene poisoning. In order to reduce benzene stimuli, based on the experience and reports of our peers, after years of exploration and practice, we have used n-butanol for dehydration and transparency since 1980.