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[Objective] To establish HPLC finger-prints of one year,three-year and five-year RADIX PSAMMOSILENES,and provide scientific,reasonable basis for the identification and quality control of it.[Method] Agilent Zorbax SB C-18 column(150.0 mm×4.6 mm,5 μm),mobile phase of acetonitrile(A) and 0.1% phosphoric acid(B),gradient elution process as follows:0-15 min,15%-20% A;5-45 min,20%-45% A;45-75 min,45%-85% A;75-80 min,85% A.Column temperature of 25 ℃,flow rate of 0.5 ml/min,detective wavelength of 210 nm.3 HPLC finger-prints were obtained under the optimum HPLC conditions,and the chromatographic peak of purified RADIX PSAMMOSILENES was as reference.[Result] HPLC finger-print method was simple and with good reproducibility,which was suitable for the identification of one year,three-year and five-year RADIX PSAMMOSILENES.[Conclusion] This study would provide reasonable and scientific basis for the identification and quality control of RADIX PSAMMOSILENES.
[Objective] To establish HPLC finger-prints of one year, three-year and five-year RADIX PSAMMOSILENES, and provide scientific, reasonable basis for the identification and quality control of it. [Method] Agilent Zorbax SB C-18 column mm × 4.6 mm, 5 μm), mobile phase of acetonitrile (A) and 0.1% phosphoric acid (B), gradient elution process as follows: 0-15 min, 15% -20% -45% A; 45-75 min, 45% -85% A; 75-80 min, 85% A. Column temperature at 25 ° C, flow rate of 0.5 ml / min, prints were obtained under the optimum HPLC conditions, and the chromatographic peak of purified RADIX PSAMMOSILENES was as reference. [Result] HPLC finger-print method was simple and with good reproducibility, which was suitable for the identification of one year, three-year and five-year RADIX PSAMMOSILENES. [Conclusion] This study would provide reasonable and scientific basis for the identification and quality control of RADIX PSAMMOSILENES.