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目的探讨免疫球蛋白重链基因重排现象应用于石蜡包埋非何杰金氏淋巴瘤（NHL）病理诊断及鉴别诊断中的意义，分析影响实验结果的可能因素。方法应用B细胞性淋巴瘤细胞株Raji作为阳性对照，免疫球蛋白重链V区及J区特异性引物，单轮PCR扩增方法对存档的29例恶性淋巴瘤（B细胞性对例，T细胞性2例），6例淋巴组织反应性增生，2例上皮性肿瘤石蜡包埋组织进行了研究。结果70．4％（19／27）的B细胞性淋巴瘤免疫球蛋白重链基因存在重排现象，并均表现为单克隆性。而T细胞性淋巴瘤以及非淋巴瘤性病变无重排现象。本研究所用方法免疫球蛋白重链基因重排的阳性检出率低于半筑巢式peR扩增（80％）。结论免疫球蛋白重链基因重排在B细胞性淋巴瘤具有特异性，能够用于该类疾病诊断及鉴别诊断中。
Objective To investigate the significance of immunoglobulin heavy chain gene rearrangement in the pathological diagnosis and differential diagnosis of paraffin-embedded non-Hodgkin’s lymphoma (NHL) and to analyze the possible factors influencing the experimental results. Methods Raji was used as a positive control, immunoglobulin heavy chain V region and J region specific primers. A single PCR amplification assay was performed on 29 cases of malignant lymphoma (B cell pairs, T 2 cases of cell), 6 cases of lymphoid reactive hyperplasia, 2 cases of epithelial tumor paraffin-embedded tissue were studied. Results 70.4% (19/27) of B-cell lymphoma immunoglobulin heavy chain gene rearrangement phenomenon, and showed a monoclonal. T-cell lymphoma and non-lymphoma lesions without rearrangement. The method used in this study immunoglobulin heavy chain rearrangement of the positive detection rate was lower than the semi-nesting peR amplification (80%). Conclusion Immunoglobulin heavy chain gene rearrangement is specific in B cell lymphoma and can be used in the diagnosis and differential diagnosis of such diseases.