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[Objective]This paper explains screening the optimal condition for extracting total flavonoids from the leaves of Rubus coreanus. [Methods]With the yield of total flavonoids content set to be the indicator,4 factors,concentration of extraction agent,solid-liquid ratio,refluxing temperature and extraction duration,were selected by means of orthogonal array design in order to carry out L 9( 34) orthogonal array design. And the content of total flavonoids was determined by spectrophotometry. [Results] The conditions of optimum extraction process were 70%- concentration ethyl alcohol,1∶ 10( g / mL) solid-liquid ratio,80 ℃ of refluxing temperature,and 3 h of extraction duration. Under these conditions,the content of total flavonoids extracted from Rubus coreanus leaves could be 44. 931 3 mg / g,far more than 202. 79 mg /100 g that was reported in the previous documents. [Conclusions] It was verified by the experiment that total flavonoids extracted from of Rubus coreanus leaves by this process was of good stability and reproductibility and had a high rate of sample recycling and certain feasibility.
[Objective] This paper explains the optimal condition for extracting total flavonoids from the leaves of Rubus coreanus. [Methods] With the yield of total flavonoids content set to be the indicator, 4 factors, concentration of extraction agent, solid-liquid ratio, refluxing temperature and extraction duration, were selected by means of orthogonal array design in order to carry out L 9 (34) orthogonal array design. And the content of total flavonoids was determined by spectrophotometry. [Results] The conditions of optimum extraction process were 70 % - concentration ethyl alcohol, 1:10 (g / mL) solid-liquid ratio, 80 ° C of refluxing temperature, and 3 h of extraction duration. 931 3 mg / g, far more than 202. 79 mg / 100 g that was reported in the previous documents. [Conclusions] It was verified by the experiment that total flavonoids extracted from of Rubus coreanus leaves by this process was of good stability and reproductibility and had a high rate of sample recycling and certain feasibility.