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以 1个水稻籼粳交 (圭 6 30 0 2 4 2 8)来源的DH群体为材料 ,利用 1张含有 2 32个标记的RFLP连锁图谱和基于混合线性模型的定位软件QTLMapper1 0对水稻收获指数及生物量、籽粒产量、库容量和株高 5个性状进行QTL分析 ,共检测到 2 1个主效应QTLs和 9对上位性互作位点。其中 ,控制籽粒产量的 3个QTLs合计贡献率为 4 2 % ,LOD值为 7 10 ;这 3个QTLs或者与收获指数的QTL同位 ,或者与生物量的QTL同位 ,且加性效应的方向一致 ,从而揭示了“籽粒产量 =生物量×收获指数”的遗传基础所在。控制收获指数的 4个QTLs合计贡献率为 4 6 % ,LOD值为 10 3;控制生物量的 4个QTLs合计贡献率为 6 4 % ,LOD值为 14 0 9;收获指数的 4个QTLs与生物量的 4个QTLs均不同位。因此 ,通过基因重组 ,可能实现控制收获指数和生物量的增效基因的聚合 ,由此获得收获指数和生物量“双高”的基因型。检测到 5个株高QTLs,其合计贡献率为 6 4 % ,LOD值为 11 6 2 ;其中 ,有 3个效应较小的QTLs与生物量、库容量和 或籽粒产量QTLs同位 ,且同位QTLs的加性效应方向一致 ;未发现株高QTLs与收获指数QTLs的同位性。由此表明 ,株高与“源 流 库”概念中的“源”和“库”在遗传上有一定程度的关联 ,而与“流”无关联。此外还发现 ,在上述同位性QTL
A DH population derived from indica-japonica rice (Kyu 6 30 0248 8) was used as experimental material, and a QTLMapper1 0 mapping system with 2 32 markers and a mapping software based on a mixed linear model was used to determine the rice harvest index QTL analysis of 5 traits including biomass, grain yield, storage capacity and plant height were performed. A total of 21 QTLs and 9 pairs of epistasis were detected. Among them, the three QTLs controlling the grain yield contributed 42% of the total and the LOD value was 7 10. These three QTLs were either located in the same position with the QTL of the harvest index or in the same position with the QTL of biomass with the same additive effect , Revealing the genetic basis of “grain yield = biomass × harvest index”. The total contribution rate of four QTLs controlling the harvest index was 46% and the LOD value was 10 3. The total contribution rate of four QTLs controlling biomass was 64% and the LOD value was 14 0. The four QTLs of harvest index The four QTLs for biomass were all different. Therefore, by gene recombination, it is possible to achieve the polymerization of the synergistic genes that control the harvest index and the biomass, thereby obtaining the genotype of “double high” for harvest index and biomass. Five plant height QTLs were detected, with a total contribution rate of 64% and a LOD value of 11 6 2. Among them, three QTLs with smaller effect were co-located with QTLs of biomass, storage capacity and grain yield, and QTLs The additive effect of the same direction; no QTLs plant height and harvest index QTLs of the same sex. Thus, plant height is genetically related to “source” and “pool” in the concept of “source reservoir”, but not to “flow”. Also found in the above isochronous QTLs