凝胶过滤法[(Gel filtration),又称凝胶层析法(Gel Chromatography)、凝胶渗透层析法(GelPermeationChromatography)、分子筛层析法(Molecular Sieve Chromatography)、排阻层析法(Exclusion Chromatography)或限制扩散层析法(Restricted Diffusion Chromatography)],是近十几年发展起来的一种分离方法。凝胶本身又称分子筛,以表示它可以把分子按大小不同进行分离,好象过筛可以把大分子与小分子分开一样。但要注意,这是一种凝胶分子筛,与另一种分子筛(硅铝酸盐型)不一样,后者是无机物质,多用于气体及一些小分子物质的分离,而凝胶是有机物制成,是在液相中用于蛋白质、多糖及其它一些较大分子物质的分离和提纯。将凝胶颗粒在适宜溶剂中浸泡,使充分吸液膨 Gel filtration, also known as Gel Chromatography, Gel Permeation Chromatography, Molecular Sieve Chromatography, Exclusion Chromatography ) Or Restricted Diffusion Chromatography] is a separation method developed in recent ten years. The gel itself, also known as molecular sieve, indicates that it can separate molecules according to their size, as if sieving separates the macromolecule from the small molecule. Note, however, that this is a gel molecular sieve, which is not the same as another molecular sieve (aluminosilicate type), which is an inorganic substance, mostly used for the separation of gases and some small molecules, and the gel is organic Into the liquid phase is used for the separation and purification of proteins, polysaccharides and other larger molecules. Soak the gel particles in a suitable solvent to allow full swellability