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目的 :旨在探讨纹状体神经干细胞 (striatum neuralstemcells ,strNSCs)的培养及分化鉴定方法。方法 :选择性分离纹状体的神经干细胞 ,体外培养、扩增和诱导分化 ,并采用免疫荧光细胞化学检查鉴定。结果 :从胚鼠纹状体分离的细胞具有连续克隆能力 ,绝大多数细胞表达巢蛋白。诱导分化后的细胞表达成熟神经细胞和星形胶质细胞特异性的蛋白。结论 :用此方法分离的细胞具有自我更新、增殖和分化潜能 ,具备中枢神经系统干细胞的一般特征。
Objective: To investigate the culture and differentiation of striatum neural stem cells (strNSCs). Methods: NSCs isolated from the striatum were isolated, cultured, expanded, differentiated and induced by immunofluorescence cytochemistry. Results: The cells isolated from the striatum of the embryonic rat have the ability of continuous cloning. Most of the cells express nestin. The differentiated cells are induced to express mature neuronal and astrocyte-specific proteins. Conclusion: The cells isolated by this method have the potential of self-renewal, proliferation and differentiation, and possess the general features of CNS stem cells.