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目的：观察人鼻咽癌细胞用电穿孔技术进行体外基因转染所需的转染电击条件及EBV－LMP基因的转染表达。方法：以人高分化鼻咽癌细胞株（CNE1）为对象，用电穿孔基因转染技术，观察不同电压及电容条件电击癌细胞对其存活率的影响；将重组EBV－LMP表达质粒转染CNE1细胞，以载体质粒转染及未经转染的CNE1细胞为对照。结果：CNE1癌细胞存活率与电容及电压值的大小成反比。CNE1细胞转染较合适条件是电压值600～1200V／cm与电容250～25μF，癌细胞存活率为56.1％～70.2％。将重组EBV－LMP表达质粒转染CNE1细胞，获得LMP稳定高表达克隆。结论：电穿孔基因转染技术可用于人高分化鼻咽癌细胞（CNE1）进行基因转染。采用该技术建立了表达EBV－LMP的人鼻咽癌细胞模型。
OBJECTIVE: To observe the conditions of transfection electroporation and the transfection of EBV-LMP gene in human nasopharyngeal carcinoma cells using electroporation technology. METHODS: Human well-differentiated nasopharyngeal carcinoma cell line (CNE1) was used as an object, electroporation gene transfection technique was used to observe the effect of electroporation on the survival rate of cancer cells under different voltage and capacitance conditions; transfection of recombinant EBV-LMP expression plasmid CNE1 cells were compared with CNE1 cells transfected with non-transfected vector plasmids. RESULTS: The survival rate of CNE1 cancer cells was inversely proportional to the size of the capacitance and voltage. The appropriate conditions for CNE1 cell transfection are voltage values of 600-1200V/cm and capacitance of 250-25μF. The survival rate of cancer cells is 56.1%-70.2%. The recombinant EBV-LMP expression plasmid was transfected into CNE1 cells to obtain a stable high-expression clone of LMP. CONCLUSION: The electroporation gene transfection technique can be used for gene transfection in human highly differentiated nasopharyngeal carcinoma cells (CNE1). Using this technique, a human nasopharyngeal carcinoma cell model expressing EBV-LMP was established.