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目的 研究体外高温 (HT)和137铯对胞质分裂阻滞的外周血淋巴细胞 (PBL)微核 (MN)产生的影响。方法 收集 6例分区放疗 (xRT)前和放疗中的肿瘤患者外周血 ,全血培养在 43.5℃下处理6 0min后 ,以137铯射线照射 ;对照为同患者的外周血。暴露于射线后在 37℃孵育 6 0min ,以PHA刺激淋巴细胞 ,培养 44h时应用细胞松驰素B ,72h时收集淋巴细胞 ,吉姆萨染色测定MN率。结果 高温 (4 3.5℃ )能使未照射淋巴细胞的MN率 (M±SE)明显提高 ,从 15 .6± 2 .8(37℃ )提高到 39.7± 10 .9。患者xRT前或xRT中 ,当淋巴细胞用高温 (4 3 .5℃ )处理联用体外照射时 ,MN率用线性方程Y =C +αD评价。结论 胞质分裂阻滞的淋巴细胞MN率测量结果表明 ,单纯 43 .5℃高温可诱导DNA损伤 ,并提高射线诱导的细胞遗传学损伤 ,且应用HT可损害接受放疗肿瘤患者的T细胞功能。
Objective To study the effects of high temperature (HT) and 137铯 on the production of micronuclei (MN) in peripheral blood lymphocytes (PBL) after cytokinesis. Methods The peripheral blood was collected from 6 patients with tumor before and during radiotherapy (xRT). The whole blood was cultured at 43.5°C for 60 min and irradiated with 137 铯. The control was peripheral blood of the same patient. After exposure to radiation, they were incubated at 37°C for 60 min. PHA-stimulated lymphocytes were used. Cytolysin B was applied at 44 h, and lymphocytes were collected at 72 h. The MN rate was determined by Giemsa staining. Results High temperature (4 3.5°C) could significantly increase the MN rate (M±SE) of unirradiated lymphocytes from 15.6±2.8 (37°C) to 39.7±10.9. In patients before xRT or in xRT, when lymphocytes were treated with high temperature (43.5°C) in combination with extracorporeal irradiation, the MN rate was evaluated using the linear equation Y = C + αD. Conclusion The measurement of lymphocyte MN rate in cytokinesis-inhibiting lymphocytes indicates that pure 43.5°C high temperature can induce DNA damage and increase radiation-induced cytogenetic damage, and the use of HT can impair T-cell function in patients undergoing radiation therapy.