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目的:观察金刚烷胺修饰物(NAM)对禽流感病毒感染小鼠免疫功能的影响,为抗禽流感病毒新药NAM的开发提供实验依据。方法:采用禽流感病毒(H5N1)感染小鼠模型,实验分正常对照组(NC)、模型组(PC)、金刚烷胺阳性药物组(AMA)及NAM25、50、100和200mg·kg-1剂量组。测定小鼠胸腺指数、脾指数、刺激指数(SI)、NK细胞杀伤活性和干扰素(IFN)活性。结果:NAM100mg·kg-1剂量组平均脾指数高于PC组(P<0.05);NAM各剂量组平均胸腺指数高于PC组(P<0.05)。NAM25、100和200mg·kg-1剂量组的刺激指数高于PC组(P<0.05);NAM100和200mg·kg-1剂量组刺激指数高于AMA组(P<0.05)。NAM100和200mg·kg-1剂量组NK细胞杀伤活性高于PC组(P<0.05);NAM25、100和200mg·kg-1剂量组NK细胞杀伤活性高于AMA组(P<0.05)。NAM50、100和200mg·kg-1剂量组IFN活性高于PC组(P<0.01);NAM50、100和200mg·kg-1剂量组IFN活性高于AMA组(P<0.05)。结论:NAM可刺激禽流感病毒感染小鼠脾细胞和胸腺细胞增殖,对T淋巴细胞转化功能、NK细胞杀伤活性及IFN免疫活性均有不同程度的促进作用。
Objective: To observe the effect of amantadine modification (NAM) on the immune function of mice infected with avian influenza virus and provide experimental evidence for the development of NAM against new bird flu virus. Methods: The mouse model was infected with the bird flu virus (H5N1). The experiment was divided into normal control group (NC), model group (PC), amantadine positive group (AMA) and NAM25, 50, 100 and 200 mg · kg- Dose group. The mouse thymus index, spleen index, stimulation index (SI), NK cell killing activity and interferon (IFN) activity were determined. Results: The average spleen index of NAM100mg · kg-1 dose group was higher than that of PC group (P <0.05). The mean thymus index of each dose group of NAM was higher than that of PC group (P <0.05). The stimulation index of NAM25, 100 and 200 mg · kg-1 dose groups was higher than that of PC group (P <0.05). The stimulation index of NAM100 and 200 mg · kg-1 dose groups was higher than that of AMA group (P <0.05). The killing activity of NK cells in NAM100 and 200 mg · kg-1 dose groups was higher than that in PC group (P <0.05). The killing activity of NK cells in NAM 25, 100 and 200 mg · kg-1 dose groups was higher than that of AMA group (P <0.05). The IFN activity of NAM50, 100 and 200 mg · kg-1 dose groups was higher than that of PC group (P <0.01). The activity of IFN in NAM50, 100 and 200 mg · kg-1 dose groups was higher than that of AMA group (P <0.05). CONCLUSION: NAM stimulates the proliferation of splenocytes and thymocytes in mice infected with avian influenza virus, and promotes the transformation of T lymphocytes, the cytotoxicity of NK cells and the immunological activity of IFN.