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目的:探讨生存素survivin基因启动子区-31C/G单核苷酸多态性与中国华南地区散发性结直肠癌(CRC)易感性的关系。方法:采用聚合酶链反应-限制性片段长度多态性法(PCR-RFLP)检测华南地区711例健康人和702例CRC的survivin基因-31C/G位点单核苷酸多态性。结果:结直肠癌患者CC基因型的频率明显高于对照人群(36.5%vs26.2%,2=17.89,P<0.01),与CC基因型相比,CG、GG基因型和等位基因G携带者的CRC发病风险分别显著下降至0.61倍(95%CI=0.46-0.80,P<0.01)、0.52倍(95%CI=0.38-0.71,P<0.01)和0.58倍(95%CI=0.45-0.74,P<0.01)。结论:survivin基因-31C/G多态与CRC发病风险有关,-31G变异基因型是中国南方人群散发性结直肠癌独立保护因素。
Objective: To investigate the relationship between -31C / G single nucleotide polymorphism of survivin gene promoter region and susceptibility to sporadic colorectal cancer (CRC) in South China. Methods: Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) was used to detect single nucleotide polymorphisms at -31C / G of survivin gene in 711 healthy people and 702 CRC patients in South China. Results: The frequency of CC genotype in patients with colorectal cancer was significantly higher than that in control subjects (36.5% vs26.2%, 2 = 17.89, P <0.01). Compared with CC genotypes, genotypes of CG and GG and allele G The risk of CRC in carriers was significantly decreased to 0.61 times (95% CI = 0.46-0.80, P <0.01), 0.52 times (95% CI 0.38-0.71, P <0.01) and 0.58 times -0.74, P <0.01). Conclusion: The -31C / G polymorphism of survivin gene is associated with the risk of CRC. The -31G variant genotype is an independent protective factor for sporadic colorectal cancer in southern China.