论文部分内容阅读
目的探讨预防性给予重组人红细胞生成素(rhEPO)对神经病理性疼痛大鼠模型机械、热痛觉高敏的影响及可能的机制。方法雄性SD大鼠30只随机均分成三组。A、B组切断L5脊神经建立大鼠神经病理性疼痛模型;C组为假手术对照。术前1d,A组腹腔注射rhEPO 5000U/kg,连续7d;B组腹腔注射生理盐水作为模型对照。采用von Frey仪测定各组0、3和7d机械缩足反射阈值;术后第7天用ELISA法测定大鼠L5脊髓组织TNF-α、IL-1β、IL-6和IL-10表达。结果与C组相比,A、B组大鼠L5脊神经损伤术后,术侧均出现明显的机械痛阈下降(P<0.01);但A组大鼠的机械痛高敏行为较B组明显缓解(P<0.01)。与C组比较,大鼠脊髓TNF-α、IL-1β、IL-6和IL-10表达明显增高(P<0.01);与B组比较,A组脊髓组织IL-6水平明显降低,而IL-10水平明显升高(P<0.01)。结论 rhEPO能预防模型大鼠神经病理性疼痛的发生,其效应与减少促炎细胞因子和增加抗炎细胞因子释放有关。
Objective To investigate the effect and possible mechanism of prophylactic administration of recombinant human erythropoietin (rhEPO) on mechanical and thermal hyperalgesia in a rat model of neuropathic pain. Methods Thirty male SD rats were randomly divided into three groups. A, B group cut off the L5 spinal nerve neuropathic pain model rats; C group sham control. One day before operation, group A received intraperitoneal injection of 5000 U / kg rhEPO for 7 days. Group B received intraperitoneal injection of saline as model control. The mechanical contralateral reflex threshold at 0, 3, and 7 days was measured by von Frey instrument. The expression of TNF-α, IL-1β, IL-6 and IL-10 in rat spinal cord L5 spinal cord was measured by ELISA method on the 7th day after operation. Results Compared with group C, the mechanical pain threshold decreased obviously in group A and B after L5 spinal nerve injury (P <0.01). However, the mechanical hyperalgesia in group A was significantly relieved compared with group B (P <0.01). Compared with group C, the expression of TNF-α, IL-1β, IL-6 and IL-10 in spinal cord of rats increased significantly (P <0.01) -10 levels were significantly higher (P <0.01). Conclusion rhEPO can prevent the development of neuropathic pain in model rats, and its effect is related to the reduction of pro-inflammatory cytokines and the increase of anti-inflammatory cytokines release.