Aim:To study the effect of adenovirus_5-mediated human hepatocyte growth fac-tor(Ad_5-HGF) transfer on post-infarct heart failure in a swine model.Methods:Twelve young Suzhong swine were randomly divided into 2 groups:the Ad_5-HGFgroup (n=6) and the null-Ad_5 group (n=6).Four weeks after left anterior descend-ing coronary artery (LAD) ligation,Ad_5-HGF was transferred into the myocardiumvia the right coronary artery.Coronary angiography and gated cardiac perfusionimaging were performed at the end of 4 and 7 weeks after LAD ligation,respectively,to evaluate collateral artery growth and cardiac perfusion.Then all animals werekilled,the expression of HGF and α-smooth muscle actin (α-SMA) were evaluatedby enzyme-linked immunosorbent assay and immunohistochemistry.Results:Compared with the null-Ad_5 group,higher expression of human HGF was ob-served in the myocardium in the Ad_5-HGF group (109.3±7.8 vs 6.2±2.6,t=30.685,P<0.01).The left ventricular ejection fraction was higher in the Ad_5-HGF groupthan in the null-Ad_5 group (43.9±4.3 vs 30.4±2.8,t=6.514,P<0.01).From the 4thweek to the 7th week after operation,left ventricular end systolic volume (42.1±3.0vs 31.0±4.9,t=12.800,P<0.01) and left ventricular end diastolic volume (62.2±4.2 vs55.0±4.8 t=13.207,P<0.01) were improved in the Ad_5-HGF group.Cardiac perfu-sion was significantly improved in the Ad_5-HGF group.In the Ad_5-HGF group,growth of collateral arteries was obviously greater (average rank sum 9.17 vs 3.83,n=6,u=-2.687,P<0.01),and the number of α-SMA~+ vessels/mm~2 was significantlygreater (56.1±4.2 vs 16.4±3.5,t=17.731,P<0.01) than in the null-Ad_5 group.Conclusion:High expression levels of human HGF were observed in the myocar-dium because of non-infarct-related vessel transfer.HGF can increase the numberof functional arterioles and improve collateral artery growth.HGF can improvecardiac perfusion and heart function. Aim: To study the effect of adenovirus_5-mediated human hepatocyte growth fac-tor (Ad_5-HGF) transfer on post-infarct heart failure in a swine model. Methods: Twelve young Suzhong swine were randomly divided into 2 groups: the Ad_5-HGFgroup (n = 6) and the null-Ad_5 group (n = 6) .Four weeks after left anterior descending-coronary artery (LAD) ligation, Ad_5-HGF was transferred into the myocardiumvia the right coronary artery. Coronary angiography and gated cardiac perfusionimaging were performed at the end of 4 and 7 weeks after LAD ligation, respectively, to evaluate collateral artery growth and cardiac perfusion.Then all animals werekilled, the expression of HGF and α-smooth muscle actin (α-SMA) were evaluatedby enzyme- linked immunosorbent assay and immunohistochemistry. Results: Compared with the null-Ad_5 group, higher expression of human HGF was ob-served in the myocardium in the Ad_5-HGF group (109.3 ± 7.8 vs 6.2 ± 2.6, t = 30.685, ). Left ventricular ejection fraction was higher in the Ad_ 5-HGF groupthan in the null-Ad_5 group (43.9 ± 4.3 vs 30.4 ± 2.8, t = 6.514, P <0.01) .From the 4th week to the 7th week after operation, left ventricular end systolic volume (42.1 ± 3.0 vs. 31.0 ± 4.9, t = 12.800, P <0.01) and left ventricular end diastolic volume (62.2 ± 4.2 vs55.0 ± 4.8 t = 13.207, P <0.01) were improved in the Ad_5-HGF group.Cardiac perfu-sion was significantly improved in the Ad_5-HGF group. the Ad_5-HGF group, the growth of collateral arteries was significantly greater (average rank sum 9.17 vs 3.83, n = 6, u = -2.687, + vessels / mm ~ 2 was significantly higher than those in the null-Ad-5 group.Conclusion: High expression levels of human HGF were observed in the myocar-dium because of non-infarct-related vessel transfer. HGF can increase the number of functional arterioles and improve collateral artery growth. HGF can improvecardiac perfusion and heart function.