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目的:建立HPLC法测定丹葛颈舒胶囊中丹参酮ⅡA含量的方法。方法:采用LunaC18色谱柱(4.6mm×250mm,5μm),以甲醇-水-醋酸(75∶25∶0.5)为流动相,流速为1.0mL·min-1,检测波长为270nm,柱温为室温,进样量10μL。结果:丹参酮ⅡA进样量在0.13～0.75μg范围内呈良好的线性关系,r=0.9995。精密度试验RSD为0.3%,重复性试验RSD为0.9%,平均回收率为99.85%,RSD=0.7%(n=5)。结论:该方法简便、可靠、准确,可用于该制剂的质量控制。
Objective: To establish a HPLC method for the determination of tanshinone II A in Dange Jingshu Capsules. METHODS: A Luna C18 column (4.6 mm×250 mm, 5 μm) was used. The mobile phase consisted of methanol-water-acetic acid (75∶25∶0.5), the flow rate was 1.0 mL·min-1, the detection wavelength was 270 nm, and the column temperature was room temperature. , 10 μL injection volume. Results: There was a good linear relationship between 0.13～0.75μg tanshinone IIA injection, r=0.9995. The precision test RSD was 0.3%, the repeatability test RSD was 0.9%, the average recovery was 99.85%, and the RSD was 0.7% (n=5). Conclusion: The method is simple, reliable and accurate and can be used for the quality control of the preparation.