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为探讨合成多肽体外诱导乙肝病毒抗原特异性Ｔ细胞（ＨＢＶ－ＣＴＬｓ）杀伤机制，应用免疫组化（ＳＰ法）检测ＨＢＶ－ＣＴＬｓＦａｓ配体（ＦａｓＬ）表达情况，并分析培养上清一氧化氮（ＮＯ）含量与细胞毒活性的关系。结果显示，ＨＢＶ－ＣＴＬｓ对转染ＨＢＶＤＮＡ的肝癌细胞（２．２．１５细胞）存在明显的特异性细胞毒活性，其Ｆａｓ配体表达阳性率分别为５８％（ＰｅｐⅠ）、５６％（ＰｅｐⅡ），明显高于ＣＤ３－ＡＫ细胞组（３０％，Ｐ＜０．０５），细胞培养上清ＮＯ含量动态分析显示，ＨＢＶ－ＣＴＬｓ细胞培养上清ＮＯ含量与细胞毒活性存在正相关关系（ｒ＝０．８，Ｐ＜０．０５），该结果提示通过Ｆａｓ－ＦａｓＬ介导细胞凋亡可能是ＨＢＶ－ＣＴＬｓ杀伤机制之一，ＮＯ可能参与了ＨＢＶ－ＣＴＬｓ功能的调节。
To investigate the mechanism of hepatitis B virus antigen-specific T cells (HBV-CTLs) killing induced by synthetic peptides, the expression of FasL in HBV-CTLs was detected by immunohistochemistry (SP method), and the levels of nitric oxide NO) content and cytotoxicity. The results showed that HBV-CTLs had obvious specific cytotoxic activity on HBVDNA-transfected hepatoma cells (2.2.15 cells), and the positive rates of Fas ligand expression were 58% (PepⅠ), 56% (PepⅡ) , Which was significantly higher than that in CD3-AK cell group (30%, P <0.05). The dynamic analysis of NO in cell culture supernatant showed that there was a positive correlation between NO content and cytotoxic activity in HBV- 0.8, P <0.05). The results suggest that apoptosis mediated by Fas-FasL may be one of the cytotoxic mechanisms of HBV-CTLs. NO may be involved in the regulation of HBV-CTLs function.