以花棒幼胚诱导的愈伤组织为受体材料,利用针-板电晕电场将pBI121质粒(含GUS基因和卡那霉素抗性基因)导入花棒愈伤组织,利用组织化学定位实验检测到GUS基因在受体组织中的瞬时表达。用80 mg·L-1卡那霉素筛选转基因花棒愈伤组织中含有的大豆Gy3基因,获得了38株再生植株,含大豆Gy3基因3株,再生植株经PCR、RT-PCR检测初步证实大豆Gy3基因已转入花棒植物基因组DNA中并发生转录。 The calli-plate corona electric field was used to introduce the pBI121 plasmid (containing the GUS gene and the kanamycin resistance gene) into the callus of the flower bud. The histochemical localization experiment Transient expression of GUS gene in receptor tissues was detected. Thirty-eight regenerated plants were obtained by screening the soybean Gy3 gene contained in the callus of Gansu Gamania with 80 mg · L-1 kanamycin, and three plants containing the soybean Gy3 gene were obtained. The regenerated plants were initially confirmed by PCR and RT-PCR The soybean Gy3 gene has been transcribed into the genomic DNA of the flower plant and transcribed.