将抗人肺癌单抗ＬＣ－１经０．２５．０．５、１、２、４ｍｍｏｌ／Ｌ不同浓度的二硫苏糖醇（ＤＴＴ），在３７℃作用１５～１２０分钟，得出最佳条件是将纯化ＬＣ－１ＩｇＭ与１ｍｍｏｌ／ＬＤＴＴ溶液混匀，３７℃保温６０分钟，ＬＣ－１ＩｇＭ降解为不同片段，经ＳｅｐｈａｄｅｘＧ１５０柱获得纯化的片段，该片段分子量经十二烷基硫酸钠－聚丙烯酸胺凝胶电泳鉴定为１１８０００，用ＥＬＩＳＡ法检测具有免疫活性，用￣（９９ｍ）Ｔｃ标记后注入带瘤裸鼠体内，能浓集于肿瘤病灶，显示该片段具有与人肺腺癌抗原特异结合的能力，有临床定位诊断的潜力． Anti-human lung cancer monoclonal antibody LC-1 by 0.25.0.5,1,2,4 mmol / L different concentrations of dithiothreitol (DTT), at 37 ℃ for 15 to 120 minutes, the best The condition is that the purified LC-1IgM is mixed with 1mmol / LDTT solution, incubated at 37 ° C for 60 minutes, the LC-1IgM is degraded into different fragments, and the purified fragment is obtained on a Sephadex G150 column. The molecular weight of the fragment is determined by sodium dodecyl sulfate-polyacrylic acid The results of enzyme-linked immunosorbent assay (ELISA) showed that it was immunoprecipitated by ELISA and labeled with ~ (99m) Tc before it was injected into the tumor-bearing nude mice and could accumulate in the tumor lesion, indicating that the fragment has specific binding to human lung adenocarcinoma antigen The ability to diagnose the clinical potential.