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目的观察红景天苷对氯气损伤大鼠肺通透性的干预作用,探讨其改善肺损伤的可能作用机制。方法雄性SD大鼠24只,随机分为4组,分别为阴性对照组,氯气吸入损伤组,单纯红景天苷对照组及红景天苷干预组。常规HE染色观察染毒大鼠肺组织病理损伤;Lowry’s法分别测定血浆与肺泡灌洗液中蛋白计算大鼠的肺通透指数;测定大鼠动脉血气计算肺动脉氧合指数;Western blotting检测肺组织中缺氧诱导因子α(HIF-1α)、内皮细胞生长因子(VEGF)、紧密连接蛋白Occludin与ZO-1的变化。结果损伤后3 h,可见肺泡结构破坏,肺泡腔内出现大量红细胞,肺间隔可见轻度炎细胞浸润;氯气可使大鼠肺通透指数与氧合指数显著下降(P<0.05);红景天干预组可显著下降肺组织中因损伤升高的HIF-1α、VEGF、Occludin及ZO-1蛋白含量。结论氯气这种窒息性毒剂在亚致死剂量吸入中毒后,3 h可出现肺通透性增加和肺组织缺氧,这种作用与激活肺组织中HIF-1α/VEGF通道,进而影响维持肺通透性的紧密连接蛋白Occludin与ZO-1的表达有关。红景天苷干预可改善相关因子的异常表达,有效改善氯气引起的急性肺损伤。
Objective To observe the effect of salidroside on lung permeability in rats with chlorine injury and to explore the possible mechanism of action of salidroside on lung injury. Methods Twenty-four male Sprague-Dawley rats were randomly divided into 4 groups: negative control group, chlorine inhalation injury group, salidroside control group and salidroside intervention group. Routine HE staining was used to observe the pathological damage of lung tissue in rats. Lowry’s method was used to determine the pulmonary permeability index of rats in plasma and alveolar lavage fluid respectively. The arterial blood gas was measured to calculate pulmonary arterial oxygenation index. Western blotting was used to detect lung tissue HIF-1α, VEGF, tight junction protein Occludin and ZO-1 were detected by ELISA. Results After 3 h of injury, the alveolar structure was disrupted, a large amount of erythrocytes appeared in the alveolar space, and mild inflammatory cells infiltrated in the lung space. Chloride significantly decreased the pulmonary permeability index and oxygenation index (P <0.05) Day intervention group could significantly reduce the lung tissue due to injury increased HIF-1α, VEGF, Occludin and ZO-1 protein content. Conclusions Chlorine gas, an asphyxiating agent, can induce pulmonary permeability and lung hypoxia 3 h after sublethal inhalation. This action can activate HIF-1α / VEGF channel in lung tissue and further affect the maintenance of lung function Occludin, a tight-junction protein, is involved in the expression of ZO-1. Salidroside intervention can improve the abnormal expression of related factors, effectively improve the chlorine-induced acute lung injury.