论文部分内容阅读
目的:观察PEDF对氧诱导的血管增生性视网膜病变动物模型视网膜新生血管的抑制作用。方法:40只7日龄的C57BL/6J新生鼠暴露在750mL/L高氧环境中,然后回到正常空气中建立氧诱导的血管增生性视网膜病变的动物模型。随机取1眼为实验眼,在出氧箱时(12d)玻璃体腔注射PEDF,另1眼为对照组,玻璃体腔注射PBS。所有小鼠均于17d处死,视网膜铺片,ADP酶染色观察视网膜血管情况。HE染色,在光学显微镜下观察并计数突破视网膜内界膜的血管内皮细胞细胞核数目。结果:与对照组相比,实验组视网膜血管分布规则,未见明显的无灌注区形成;实验组突破内界膜的内皮细胞细胞核数目(10.18±1.74)比对照组(38.89±2.98)明显减少(P<0.01) ;组织切片未见视网膜毒性及炎症反应。结论:PEDF能够有效抑制视网膜新生血管的生成。
OBJECTIVE: To observe the inhibitory effect of PEDF on retinal neovascularization induced by oxygen in animal model of angioproliferative retinopathy. METHODS: Forty seven-day-old C57BL / 6J neonatal rats were exposed to 750 mL / L hyperoxia and returned to normal air to establish oxygen-induced angiogenic retinopathy in an animal model. One eye was randomly selected as the experimental eye. PEDF was injected into the vitreous cavity in the oxygen chamber (12 days), the other one as the control group, and the vitreous cavity was injected with PBS. All mice were sacrificed at 17d, retinal patch, ADP enzyme staining observed retinal blood vessels. HE staining, observed under a light microscope and count the number of cells breaking the inner limiting membrane of the vascular endothelial cells. Results: Compared with the control group, the distribution of retinal blood vessels in the experimental group was not obvious, and the number of endothelial cells in the experimental group (10.18 ± 1.74) was significantly lower than that in the control group (38.89 ± 2.98) (P <0.01). There was no retinal toxicity and inflammatory reaction in tissue sections. Conclusion: PEDF can effectively inhibit retinal neovascularization.