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为了解水杨酸(Salicylic acid,SA)缓解轮纹病菌伤害植物的机理,用0.3 mmol L-1的SA预先处理梨叶片,3 d后接种轮纹病菌(Physalospora Piricola Nose),接种4 d后开始测定各种指标,随后每隔3 d测定1次,共5次.结果表明,经0.3 mmol L-1 SA处理后,梨叶片的超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)、抗坏血酸过氧化物酶(APX)、愈创木酚过氧化物酶(GPX)和谷胱甘肽还原酶(GR)的活性分别比对照组(CK1)提高了30.65%、25.36%、28.55%、21.11%、35.67%和26.45%.电镜观察表明,感染轮纹病菌的梨叶片,Ca2+较多分布在细胞膜内侧、细胞质及一些细胞器(叶绿体)上.经SA处理后感染轮纹病菌的梨病叶,Ca2+较多分布在细胞壁外侧及细胞间隙中,与正常梨叶片Ca2+的分布情况相似.表明较低浓度的SA可缓解轮纹病菌对梨叶片的伤害作用.
In order to understand the mechanism of salicylic acid (SA) alleviating pathogen damage by S. rotster, pear leaves were pretreated with 0.3 mmol L-1 SA and inoculated with Physalospora Piricola Nose after 3 days. The results showed that after treatment with 0.3 mmol L -1 SA, the activities of superoxide dismutase (SOD), peroxidase (POD) and malondialdehyde CAT, ascorbate peroxidase (APX), guaiacolperoxidase (GPX) and glutathione reductase (GR) were higher than that of the control group (CK1) 30.65%, 25.36%, 28.55%, 21.11%, 35.67% and 26.45% respectively.Electron microscopy showed that Ca2 + distributed more on the inside of cell membrane, cytoplasm and some organelles (chloroplast) Compared with normal pear, Ca2 + distribution of Ca2 + was more distributed in the lateral and intercellular spaces of pear leaves infected with P.lotaci, which indicated that lower concentration of SA could alleviate the damage effect of S.ruberculosis on pear leaves .