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目的:建立清热镇惊散的定性定量方法。方法:采用薄层色谱法对方中黄连、青黛进行了定性鉴别,同时采用高效液相色谱法测定了制剂中栀子苷的含量,色谱柱为迪马C18(4.6 mm×150 mm,5μm),流动相为乙腈-水(13∶87),检测波长为238 nm。结果:定性鉴别薄层色谱特征明显,含量测定栀子苷在24.58~73.74μg范围内呈良好的线性关系(r=0.9996),平均回收率为99.72%,RSD 0.98%(n=6)。结论:建立的定性定量方法可控制制剂的质量。
Objective: To establish a qualitative and quantitative method of clearing away heat shock and shaking up. Methods: The contents of geniposide in Rhizoma Coptidis and Indigo Naturalis were identified by TLC, and the content of geniposide was determined by HPLC. The chromatographic column was DiMA C18 (4.6 mm × 150 mm, 5 μm) The mobile phase was acetonitrile-water (13:87) and the detection wavelength was 238 nm. Results: The qualitative identification TLC showed that the content of geniposide was linear in the range of 24.58 ~ 73.74μg (r = 0.9996). The average recovery was 99.72% and the RSD was 0.98% (n = 6). Conclusion: The established qualitative and quantitative method can control the quality of preparation.