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目的探讨丝胶对2型糖尿病大鼠视网膜低氧诱导因子-1ɑ(HIF-1ɑ)表达的影响。方法将48只SPF级健康雄性SD大鼠依照随机数字分配方法随机分为正常对照组、糖尿病模型组、丝胶治疗组和导升明阳性对照组,每组12只。糖尿病模型组、丝胶治疗组、导升明阳性对照组大鼠均采用链脲佐菌素(STZ)连续腹腔注射3d并给予高脂饲料饮食法制备糖尿病大鼠模型;成模后分别用生理盐水、2.4g/(kg·d)丝胶溶液和0.2g/(kg·d)导升明溶液灌胃35d。苏木精-伊红染色观察各组大鼠视网膜组织的形态学变化;Western blot技术、实时荧光定量PCR技术检测各组大鼠视网膜组织HIF-1ɑ蛋白及mRNA的相对表达量。结果正常对照组大鼠视网膜组织结构正常,糖尿病模型组大鼠视网膜组织形态学出现明显的病理变化,HIF-1ɑ蛋白及mRNA相对表达量高于正常对照组(P<0.05)。导升明阳性对照组、丝胶治疗组大鼠视网膜组织形态学的病理变化减轻,HIF-1ɑ蛋白及mRNA相对表达量均低于糖尿病模型组(P<0.05)。结论丝胶可能通过下调视网膜HIF-1ɑ表达,从而对糖尿病视网膜微血管病变发挥保护作用。
Objective To investigate the effect of sericin on the expression of hypoxia inducible factor-1 (HIF-1) in the retina of type 2 diabetic rats. Methods Forty-eight SPF healthy male SD rats were randomly divided into normal control group, diabetic model group, sericin treatment group and positive control group, 12 rats in each group according to random number distribution method. Rats in diabetic model group, sericin treatment group and Dashengming positive control group were given streptozotocin (STZ) intraperitoneal injection for 3d consecutively and given high-fat diet to prepare diabetic rat model. After modeling, Saline, 2.4g / (kg · d) sericin solution and 0.2g / (kg · d) ascending solution for 35 days. The morphological changes of retina were observed by hematoxylin-eosin staining. The protein and mRNA expression of HIF-1α in retina were detected by Western blot and real-time fluorescence quantitative PCR. Results The retinal structure of normal control rats was normal. The histopathological changes of retina were observed in diabetic rats. The relative expression of HIF-1α protein and mRNA was higher than that of normal control rats (P <0.05). The results showed that the histopathological changes of retinal tissue in the seronegative treatment group were alleviated, and the relative expression of HIF-1α protein and mRNA in the seronegative treatment group was lower than that in the diabetic model group (P <0.05). Conclusion Sericin may play a protective role in diabetic retinal microangiopathy by down-regulating the expression of HIF-1ɑ in retina.