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本文在国内首次报道了北黄海硷渣及三类废弃物试验倾倒区水和沉积物样品中硝化细菌和硫化细菌数量的水平和垂直分布,分析了菌属组成和季节变化。 硫化细菌在水样和沉积物样品中的检出率均为100％。但数量此本海区异养细菌少1个数量级,在表层海水中平均为4.8×103个·L-1;表层沉积物中平均为1.4×103个·L-1。而且,分离的硫化细菌菌株都隶属于硫杆菌属(Thiobacillus sp.)。 硝化细菌在该海区检出率很低,表层水样中检出率为26.9％;表层沉积物为22.2％。前者大多在50个·L-1以下;后者一般少于250个·L-1。本海区的硝化细菌(不包括亚硝化细茵)由硝化球菌属(Nitrococcus sp.)、硝化刺菌属(Nitrospina sp.)和硝化杆菌属(Nitrobacter sp.)三个菌属组成。以检出率最高的春季为代表,硝化细菌的优势菌属无论在水样和沉积物样品中均为硝化杆菌属。此外,硝化细菌的数量在一定程度上受水温和亚硝酸盐含量等的影响。
This paper reports for the first time in China the horizontal and vertical distributions of nitrifying bacteria and sulfide bacteria in water and sediment samples of the North Yellow Sea alkali residue and the three types of waste dumps, and analyzes the composition and seasonal variation of the bacteria. Vulcanization bacteria in water samples and sediment samples were 100% detection rate. However, the number of heterotrophic bacteria in this area is less than one order of magnitude, with an average of 4.8 × 103 · L-1 in surface seawater and 1.4 × 103 · L-1 in surface sediments. Moreover, all of the isolated strains of the sulfide bacteria belong to the genus Thiobacillus sp. The detection rate of nitrifying bacteria in the sea area is very low. The detection rate of surface water samples was 26.9% and that of surface sediments was 22.2%. The former is mostly below 50 · L-1; the latter is generally less than 250 · L-1. The nitrifying bacteria (excluding nitrosated bacteria) in this area consist of three genera of Nitrococcus sp., Nitrospina sp. And Nitrobacter sp. Represented by spring with the highest detection rate, the dominant bacterial species of nitrifying bacteria are both Nitrobacter both in water samples and sediment samples. In addition, the number of nitrifying bacteria is influenced to some extent by water temperature and nitrite content.