论文部分内容阅读
背景:近年的移植实验表明,移植视网膜内能合成相关的神经活性物质,但移植视网膜内神经活性物质阳性神经元的生长发育情况尚不清楚。目的:观察移植视网膜神经元的分化、发育,神经活性物质的生物合成及其与周围环境、视觉中枢的联系,以探讨神经组织的发育规律及其再生和可塑性。设计:以动物为研究对象,观察对比研究。单位:两所大学的组胚教研室。材料:实验于2002-08/2003-03在广州医学院实验动物中心完成。选择健康SD大鼠96只,雌雄不限。干预:用胚14dSD大鼠视网膜移植至P1大鼠中脑左侧上、下丘间,同时摘除P1鼠右眼,术后第9天移植视网膜的发育时间与生后第1天正常视网膜相当,记为TP1,余类推,应用组织化学方法显示神经元的发育和分化。主要观察指标:移植视网膜和移植视网膜神经元的发育及形态观察。结果:移植视网膜具有正常视网膜的各层结构和相似的生长发育规律,并能合成相关的神经活性物质。移植视网膜内一氧化氮合酶阳性神经元开始出现于TP4,TP12时阳性神经元数量达到高峰,TP22后阳性神经元数目维持在较低的水平。结论:胚胎视网膜脑内移植后能存活且保持原有的生长发育规律,具有正常视网膜一样的组织结构特征。
BACKGROUND: Transplantation experiments in recent years show that intraretinal retinal synthesis can be associated with neuroactive substances, but the growth and development of neurons in the retina are not clear. OBJECTIVE: To observe the differentiation and development of transplanted retinal neurons, the biosynthesis of neuroactive substances and their relationship with the surrounding environment and visual center so as to explore the developmental rules of neural tissues and their regeneration and plasticity. Design: The animal as the research object, observing the comparative study. Unit: Two groups of embryos in the Department. Materials: The experiment was performed at Experimental Animal Center of Guangzhou Medical College from August 2002 to March 2003. Choose 96 healthy SD rats, male or female. Intervention: Embryo 14dSD rat retina was transplanted to the left middle of the middle cerebral artery of P1 rats, the inferior colliculus and the right eye of P1 rats were removed at the same time. The developmental time of the retina transplanted on the 9th day after surgery was the same as that of the normal retina on the first day after birth, Recorded as TP1, and so on analogy, the application of histochemical methods to show the development and differentiation of neurons. MAIN OUTCOME MEASURES: Development and morphology observation of retinal and transplanted retinal neurons. RESULTS: The transplanted retina possessed the normal retina structure and similar growth and development rules, and could synthesize related neuroactive substances. Nitric oxide synthase positive neurons in retina began to appear in TP4, TP12 positive neurons peaked, TP22 positive neurons maintained at a low level. Conclusion: Embryonic retina can survive after intracerebral transplantation and maintain the original law of growth and development, with the same normal retinal tissue structure.