论文部分内容阅读
1971年,美国斯坦福大学Goldstein第一个利用同位素~(14)C-左吗伦与小鼠脑匀浆的立体特异性结合来显示鸦片受体,但由于存在大量的非特异性结合,且同位素标记物的比活性低,所测得的立体特异性结合只占全部结合的2％。1973年,美国约翰-霍浦金斯大学Snyder与Pert,纽约州立大学Simon及瑞典乌帕塞拉大学Terenius,分别用~3H-纳洛酮、~3H-埃托芬(etorphine)、~3H-双氢吗啡,同时确证了鸦片受体的存在,为研
In 1971, Goldstein, the first American Stanford University, used the stereospecific binding of isotope ~ (14) C-dexamethasone to mouse brain homogenates to display opioid receptors. However, due to the large number of nonspecific bindings, isotope labeling The specific activity of the material is low, and the measured stereospecific binding only accounts for 2% of the total binding. In 1973, Snyder and Pert of the Johns Hopkins University in the United States, Simon of New York State University and Terenius of Uppsala University in Sweden were treated with ~ 3H-naloxone, ~ 3H-etorphine, ~ 3H- Dihydromorphine, while confirming the existence of opioid receptors for research