目的由于抗鼠疫杆菌毒力抗原VW因子血清的制作较难,因而除T.W.Burrows的研究室外,是根据细菌于草酸镁琼脂培养基上,37℃及26℃培养下菌落形成能力的差异来判定V W抗原的有无。Gemski等曾分离了支配结肠炎耶尔森氏菌及假结核耶尔森氏菌产生V W的42×10~6道尔顿的质粒,但限制酶切断的模式是不同的。另一方面,塚野 Purpose Since it is harder to produce anti-Plaguecaria virulence antigen VW factor serum, the difference in colony-forming ability of bacteria on the magnesium oxalate agar medium at 37 ° C and 26 ° C is judged outside of the TWBurrows study to determine whether the VW Is there any antigen? Gemski et al. Have isolated 42 × 10 ~ 6 Dalton plasmid that controls Yersinia enterocolitica and Y. pseudotuberculosis to produce V W, but the mode of restriction enzyme cutting is different. On the other hand, Tsukano